Coding
Part:BBa_K2587003:Design
Designed by: Ylenia Longo Group: iGEM18_Duesseldorf (2018-09-15)
luxR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 196
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6
Illegal BsaI.rc site found at 637
Design Notes
The sequence contains TypeII S restriction sites for special use in the CIDAR toolbox.
Source
The sequence was obtained from Genbank and codon optimised for E.coli.